Anirban Guha

Trees provide shade, but have you ever considered trees themselves needing shade? Our lab is seeking to answer this question. Anirban Guha, who is leading this effort in our lab, was able to sit down with me and answer some questions about the experiment. He joined the lab in April 2019 as a post-doctoral scholar. The experiment is attempting to determine how the trees respond to different light conditions over a period of two to three years with the use of shade nets to manipulate the environmental conditions. The lab records daily, weekly, and monthly results, and will record the yearly results when the time comes. We already know that citrus responds better in partial shade conditions, which improve yield and yield quality and photosynthesis and water status. We think that full sun has an especially bad effect on HLB trees. Anirban explained that the infected plants often cannot take in the full force of Florida sunlight; it provides them with more energy than they have the capacity to process. HLB also stunts root growth, which becomes even more of a problem when high light conditions demand more water and nitrogen than can be taken up by the roots.

The lab is testing whether shading the trees allows them to conserve more energy and require less water and nitrogen, which would help balance their functioning with the disease. Ultimately, the goal is to “[develop an] agricultural system in a way that could modify the environmental cues, and that can lead to better fitness of the plant to help sustain yield and maintain better physical performance.”

The main recipients of this experiment are scientists and citrus growers; Anirban thinks these two groups believe they have different reasons for caring about the results, but he believes their goals are actually similar and the knowledge they seek is complementary. Whether results are sought for economic reasons or a research quest, the ultimate goal is to see the trees become healthier and create more fruit yield—something both the scientific and agricultural communities can agree upon. 

Anirban takes this collaborative approach in his work life as well. The physiology lab collaborates with other CREC labs to study and test infected trees. Their results often work together to create healthier trees. For example, the entomology lab provides information on how insects spread HLB. He desires for more scientists of different disciplines to work together to achieve “functional collaborative research,” which can help the scientific community locally and worldwide. Along with scientists working together to achieve more, he also wants his research to be holistic. He wanted to study trees not just at a cellular level, but “from leaf to whole plant.” After completing his PhD in India, he found the majority of opportunities available there were for study at the cellular level. Anirban was interested in more variety and didn’t want to do what everyone else was doing. He also saw this gap in research as something he could potentially fill back home one day. He believes the study of the whole tree is important because problems tend to be linked to one another and can be better understood when a whole plant approach is taken. He enjoys his work but told me with a good-natured smile that he is not at all attached to the state of Florida and would like to return to India one day. 

Advertisements

Coordinating psyllid management with citrus flush

Because the Asian citrus psyllid stakes its reproduction on new citrus flush, there is a lot of interest in tailoring management to citrus phenology.  “Phenology” is an uncommon word, but it boils down to how plant development changes over time.  For instance the development of the spring flowering flush is a phenological process and names like “feather flush,” “popcorn,” and “full bloom” describe phenological stages.

Gene Albrigo has been involved in phenological modeling to predict flowering intensity and bloom time since well before the HLB era.  He has recently turned to using this model to help improve psyllid management in two ways: reducing psyllid reproduction on new flush through pre-emptive  psyllid management, and reducing negative impacts of insecticides on bee pollinations.  In other words his goal is to kill adult psyllids before they can lay eggs on tender new flush but not hurt pollinator bees with applications late in the flush, when flowers have emerged. This can be done by using the models he and collaborators developed and have maintained for more than 10 years.

Gene has worked with several regional growers, selecting some blocks to manage psyllids based on phenological predictions, leaving others as controls with calendar or sampling-based sprays.

2018-05-22 03.48.30
A May feather flush with psyllid damage.

Gene recently reported results from the first two years of developing this approach.  Results are positive, with reductions in adult psyllid numbers and egg-laying using the phenology-based approach, spraying once just prior to budbreak and again about 4 weeks later.  This also allowed a bloom period that was free of insecticide applications, leaving the pollinators to range at the appropriate time.  These results are promising for psyllid management during the floral flush, and I expect this approach to expand to become a standard practice.

Effects of kaolin on Asian citrus psyllid

Kaolin films are showing promising results in management of Asian citrus psyllid.  I recently presented preliminary results from our trial of Surround kaolin clay product and a Surround that we have modified with a red dye in presentations to the Polk County OJ Break and to the Citrus Research and Development Foundation research lunch.  To see the complete presentation click here.   

kaolin in field 6 mo
Six-month old trees with white kaolin.

The results are promising: Over the course of the first year after planting we saw an 78% reduction in mean psyllid numbers per tree in the white kaolin treatment.  Thus far, this has also translated in lower infection rates, with a mean of 10% infection in the white kaolin versus 25% in the foliar insecticide treatment.  These results are early, so we should be cautious about jumping to conclusions.  However, other studies have produced similar results, and this means that growers should consider kaolin as a viable practice to incorporate into their management programs.

psyllid plot 1 year
Mean Asian citrus psyllid counts based on weekly counts over the first year after planting on trees treated with Red-dyed or non-dyed (White) kaolin or with foliar insecticide. Click here for full presentation.

 

What is the right actinic light level for OJIP analysis?

Background

We recently began trying to measure OJIP, more succinctly known as “the transient rise in chlorophyll fluorescence,” on citrus plants.  We immediately ran into values that did not make intrinsic sense, with large differences between Fo and O, and with traces that did not look like the characteristic sloped steps of OJIP analysis.  We are using the Opti-Sciences OS-30p+ to collect data. We called the company, and their technicians were very helpful.  They suggested that the modulated light power should be reduced. In order to do that we did not activate photosynthesis with our modulated (far-red light).  They also recommended that we optimize the actinic light level.  Because we could not find any published systematic approach to it we decided to try to go about it in a systematic way ourselves, and share the results with anybody who might be interested.  It is interesting to note, that I had worked with OJIP of papaya for 3 years, and never noticed such problems. Thus, optimization may need to be species- and/or conditions-based.

 

Methods

We were used two (2) varieties of citrus (Murcott and Ruby Red) under two light levels: full sun and the Citrus Under Protection environment (approximately 20% shade). Leaves were selected randomly at approximately 2 to 3 meters from the ground. In the same row we put dark acclimation clips on leaves using one (1) leaf per tree. After one (1) hour of dark acclimation, we collected data for 8 different actinic light level from the lowest to the highest level: 525, 875, 1000, 2500, 3000, 3500, 4500, and 6000 µmol m-2 s-1 using the OS-30p+ fluorometer using the JIP protocol (Opti-Sciences, Hudson NH).  This was performed only once.

 

Analysis

The results were subjected to principal component analysis, including only raw data: tFm, Fo, O, J, K, I, P, S, using prcomp from R stats and the factoextra package for visualization.

 

Results

The raw data were simplified because K, Fo, and S were very positively correlated (redundant).  Thus the final results include, tFm, O, J, I, and P.  The first 2 principal components account for approximately 86% of the variance, and the third PC adds another 10% (Scree plot).   tFm and O are negatively correlated on both the first and second PCs, and I and P add a different direction of effect.

screeplot-ojip-pca

Actinic light level is the primary contributor to the variance in PC1, and a significant main effect on PC2, with no interactions with other factors (Anovas below).  In theory This makes the actinic light part easy.

screenshot-2016-11-22-11-59-04

screenshot-2016-11-22-11-59-20

Ideally we would find an actinic light level that had confidence intervals that overlapped with zero in both directions, but no dice.  The means for 1000 µmol m-2 s-1 and 2500 µmol m-2 s-1 are closest to 0 and upon visual inspection, you would think that somewhere around 1800 µmol m-2 s-1 would be very close to zero (Figure below).

light-level-pca

Another point to consider is that initial problem including large differences between Fo and O.  Light level also had a significant impact on this difference, but with an interaction with the other 2 factors.  While it is normal to have some difference between Fo and O, and because Fo is measured in the “dark” and O immediately after turning on the actinic light, you would expect delta O (Fo-O) to be slightly negative, but certainly, not 200 different (Fm being around 900), as with 6000 µmol m-2 s-1. However, at 1000 µmol m-2 s-1 the value of delta O overlaps above 0, which would make me think that perhaps this is not quite enough light, while at 2500 µmol m-2 s-1 the different varieties and locations are distinguishable, which should not necessarily be the case, as Fm and O are different estimates of the same characteristic.   Once again, neither 1000 nor 2500 µmol m-2 s-1 can be eliminated, while it appears that perhaps an intermediate light level could be more suited.

delta-o-plot

Still, delta O appears to respond toothed factors in addition to actinic light level (Analysis of variance results below).

screenshot-2016-11-22-11-58-36

Thus a delta O below 0 is not necessarily a mistake.  The only potential explanation for this that I have is that the rate of rise of the early part of the transient effects a higher O relative to Fo.  If this is true Vj should be negatively correlated to delta O.  I tested this with car.test in the basic R stats package, which uses a Pearson’s product-moment correlation and derives a p-value. The result was a correlation of -0.487, with 95% confidence interval of -0.567 to -0.399, and a p-value of <0.0001.  My conclusion is that delta O is affected by light level but also by photochemical structure of the leaf, primarily in absorption-trapping.

The overall conclusion, however, is that actinic light level affects the results of the OJIP test.  Although it is not entirely clear what the results should be, it seems that chlorophyll fluorometer companies could help us by including some actinic levels in the range of 1800 µmols m-2 sec-1.   I would welcome any comment and would like to see a more systematic approach to OJIP analysis in generally.  A discussion among those who use the test would likely lead to a more sound approach.